BIOL308 Study Guide - Taf1, Helicase, Protein Kinase

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Transcription and translation in eukaryotes occurs in separate compartments. Eukaryote pre-mrnas are subject to extensive post-transcriptional modification processing. Chromatin structure in eukaryotes limits accessibility and transcription is highly regulated. Eukaryotic rnap does not recognize binding sites and needs general tfs to help. Eukaryotic transcription has 3 different rnaps, each with different roles. The compounds were injected in mouse cell nuclei and then the transcripts produced were subject to gel electrophoresis. Certain genes were proven to be transcribed while others were not. Rna polymerase i: transcribes rrna (28s, 18s and 5. 8s rrna) Rna polymerase ii: transcribes mrna, snrnas and mirna. It is a consensus sequence which is unique to rna polymerase ii. The dephosphorylated (ii a) form is required for rnapii binding to the pic. The phosphorylated form (ii o) is required for elongation. Enhancers are genetic elements to which activators can bind and increase the ability of rnap to bind the promoter they can be found anywhere.

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