PHGY 210 Lecture Notes - Lecture 13: Open Reading Frame, Cystic Fibrosis, Centromere
Document Summary
Sickle-cell anemia oligonucleotide that encompasses mutation normal allele only hybridizes only when hg a is there mutated allele only hybridizes when there"s hg s. Taken dna samples from individuals, put on 2 separate pieces of cellulose (both membrances0. !st individual is heterozygous, put on both membranes. Mutation that removes three nucleotides is in open reading frame (remove one amino acid) > mutated form of sequence. Mutated form will have same sequence except for three sequences. Mutated can hyb. w/ normal aso and mutated aso. Screen blood samples from people for the presence of sickle-cell allele) presence of diagnostic. Want to get single cell allele: view rbcs under microscope and look for sickle shapes. Cannot see allele under microscope: denature dna in blood samples and perform aso hybridization screen, determine dna sequence of each allele using as oligo primer, thereby obtaining sequence information over.
