BIOL 205 Lecture Notes - Lecture 12: Whole Genome Sequencing, Human Genome Project, Celera Corporation
Document Summary
Table 24. 1in 2003 the human genome project completed an initial draft sequence of the human genome after ~13 years and a cumulative cost of ~ million. Costs dropped sharply over the course of project due to improvements in sequencing technology especially replacing gels with capillary electrophoresis. 600 bp of sequence possible in each run. Human genome project : government based: individual chromosomes or total genome dna, digest or shear clone into cosmids library, isolate dna from each cosmid clone re fingerprint. Clone 1 clone 2: feed fingerprint data into computer look for overlaps. Founded celera genomics: sequenced his dog, predicts that future will have everyone sequenced, sanger institute: announcement 10,000 human genomes in next 3 years. 26,588 open reading frames (# is dropping 2006 19-22,000 predicted genes. 11,226 had homology human or other organisms -orthologs. 42% of orfs have no known or predicted function! Many genes arose by duplication (retrovirus?) mrna. 60% of human protein-coding genes have multiple splice variants.
