BIOL 341 Lecture Notes - Cold Shock Response, Ice Crystals, Apoplast
Document Summary
Plants responding to cold and freezing stress. Cold shock vs cold acclimation: quick cold shock damages plant, membranes affecting fluidity. Affect ability to control what goes in and out; important in cell organization. Induced in drought, salinity and freezing tells plant there is some sort of water stress to induce plant response. When put in cold stress, can semi- quantitavly measure how much ca2+ is expressed. Increase amount of unsaturated fas can often increase cold tolerance. Prevent denaturation/ protect the tertiary structure of proteins: antioxidant: prevent radicals from disrupting cell. Hosi (e3 ligase) targets ice1. General classes of proteins associated with cold remediation: We know its defs a negative regulator. Hos1 (ubi ligase) is inhibitor of ice1 activity: hos1 = negative regulator, loss of hos1= increase cold tolerance. Overexpress it = decrease cold tolerance. In low temp, ice1 stabilized and not as susceptible to hos1. Supercooling: extracellular water freezes but intracellular does not. Cell is below zero but.