Anatomy and Cell Biology 4429A Lecture : 15. Differentiation

36 views2 pages

coffeecicada91

22 Jan 2012

School

Western University

Department

Anatomy and Cell Biology

Course

Anatomy and Cell Biology 4429A

Professor

Paul Walton

For unlimited access to Class Notes, a Class+ subscription is required.

Document Summary

Paneth cells at the very bottom of intestinal crypts secretory cell, antimicrobial peptides such as lysozyme. Much less complicated differentiation than for the leukocytes. Mesenchymal cells secrete growth factors such as wnt, these gf tell the cells they are stem cells. As they begin to move away from here the amount of wnt they see decreases. Maybe paneth cells lack wnt receptors so that is why they don t differentiate. Pax7 expression means they are capable of differentiating but they don t do it, gets turned down when they differentiate. How did we discover the genes being turned on and off in myogenesis. 5-azacytidine = cannot be methylated to keep gene expression on. This gets rid of any gene regulation, all genes are turned on. Subtractive hybridization lets you pick out what genes are being expressed. Pay attention to the red mrna necessary for making a cell a muscle cell.

Related Questions

Causality1

Learning Goal:

Interpret Causality

Fibroblasts are cells that can be isolated from the connective tissue under the skin and grown in culture. Normally these cells retain the properties of fibroblasts as they divide for many generations in culture, but if we do an experiment in which the fibroblasts are forced to take up a highly expressed copy of the MyoD gene, they rapidly differentiate to become muscle cells.

a) MyoD expression is sufficient to cause fibroblasts to differentiate to muscle cells in culture

b) MyoD expression is correlated with muscle cell differentiation in culture

c) MyoD expression is necessary and sufficient to cause fibroblasts to differentiate to muscle cells in culture

d) MyoD expression is necessary for fibroblasts to differentiate to muscle cells in culture

I understand that differential gene expression occurs in eukaryotes, where every cell contains the entire genome but differentiated cells only express particular genes important for their function. My question is in regards to prokaryotic cells. Do prokaryotic cells exhibit differential gene expression in the way that eukaryotes do? Or do they express all genes in the cell?

You have discovered a strange new enzyme that can carry out demethylation on methylated lysine R-groups as well as on 5-methylcytosine, despite the very different structures of these molecules. Using this method in conjunction with gene expression analysis, is it ever possible to differentiate whether a gene is regulated by histone methylation or by DNA methylation? If so, give an example. If not, why is this not possible?

ceruleanporpoise612

Anatomy and Cell Biology 4429A Lecture : 15. Differentiation

Document Summary

Get access

Related Documents

Anatomy and Cell Biology 4429A Lecture : Cell Cycle

Anatomy and Cell Biology 4429A Lecture Notes - Lecture 15: Basic Helix-Loop-Helix, Apoptosis, Fibroblast

BIOL 3410 Lecture Notes - Lecture 12: Retina, Micrograph, Socalled

Related Questions