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CSB429H1 Lecture 3: Lecture 3 - Jan 26.docx

Canada

Germ Cell Biology

Cell and Systems Biology

Dorothea Godt

University of Toronto St. George

Plant Molecular Biology and Biotechnology

<div></div> <p>in our online lecture we include a quote by E. O. Wilson,</p>

<p><p>Why is such a seemingly complicated scheme used for LacZ complementation (X-gal colour selection and Î±-peptide complementation), in which most of the lacZ gene is in the host chromosome and the small Î±-peptide resides in the cloning vector? In other words, why not have the entire lacZ gene in the vector? Give two reasons. [3 marks] [Clue: From the relevant lectures and the Lab Manual, based on colour selection (Lecture 4) and the sizes of recombinants (Lecture 5)]</p></p>

<p>Would you have liked to hear Mendel's lectures on his pea plants? Why or why not?</p>

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