ANAT 262 Lecture Notes - Pex5, Xpo1, Peroxin-7
Document Summary
Direction of rna transport all have trafficking aided by transporters. Mrna will not be exported if it is not spliced (also needs 5" cap and 3" poly a tail but those are less important) Transcript in nucleus contains introns which have nuclear retention signal so un- til splice they will not be shuttled to the cytoplasm. Virus has an rre which rev binds to (rev contains a nuclear export signal) so if. Crm1 binds to rev we have nuclear export. Rev protein binds to rre which has hydrophobic export signal which is recog- nized by crm1. Drug lmb interferes with crm1 functioning and causes disappearance of virus but cells die as it is not specific and prevents export of other important molecules. Creating mutant rev with non-working nes would prevent export. Looking for drug to prevent rev binding to rre. Ddx19 is the human version of dbp5p which needs to be on rna for tap to export it.