CHEM 271 Study Guide - Midterm Guide: Affinity Chromatography, Protein Purification, Ion Exchange

• School: Concordia University
• Department: Chemistry
• Course: CHEM 271
• Professor: Brandon Findlay

Document Summary

You should be able to describe how we can use centrifugation to separate cell components. You should be able to describe how salting out separates proteins. For the following techniques you should be able to describe which protein property governs separation, and how each technique works. You should also be able to apply this knowledge. You should be able to explain the salting out procedure. You should be able to describe how dialysis works. You should be able to explain the principles behind gel electrophoresis. You should be able to describe sds polyacrylamide gel electrophoresis, isoelectric focusing, and 2d gel electrophoresis. You should be able to define the isoelectric point.