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Lab 1 - Exam Review (Procedures)

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Biology (Sci)
BIOL 111
Suzanne Gray

LAB 1: The Microscope and Experimental Design Microscope: Phytoplankton: microscopic algae  Become more abundant when exposed to higher nutrient levels  Enhanced phytoplankton growth in a lack can have many negative consequences Microcosm: miniature model system Key principles of experimental design are: 1. Develop a hypothesis a. Tentative explanation for a well framed question 2. Designing an experiment with controls a. Untreated microcosm 3. Designing an experiment with replication 4. Randomizing treatments Daily Care of Microscope: 1. Wipe each lens with a Kimwipe 2. Turn off illumination and unplug cord Low Power: 3.2x objective (brown band lens) Total Magnification: magnification of eyepiece x magnification of objective x magnification of binocular tube Newsprint: letters appear upside down and backwards  Move in the direction opposite than the actual movement of the slide  Fibers that make up the paper are from wood Wet mount: 1. Drop of water 2. Place cover slide 3. No bubbles Optical section: single plane of focus in an object Plant and Animal Cells Cells contain DNA, organelles and cytoplasm which are surround by a cell membrane (plants and fungi have a cell wall) Protozoans are unicellular, while plants and animals are multicellular  Multicellular; organized into different tissues: groups of cells that function together LAB 1: The Microscope and Experimental Design  Tissues may further be grouped in a structural and functional unit called organ (group of organs working together is an organ system) Elodea Leaf  How many layers of cells? 2 layers  Uniform in size? One layer is larger than the other (smaller cells on top)  Thorn cells: project outwards making close observation easy  Chloroplasts: visible green sacs  Cytoplasmic Streaming: movement with in the cell, cytoplasm continually moving  Iodine Solution: one drop on the edge of the coverslide  Pyrenoids (starch containing bodies) inside the chloroplast can be see
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