PHGY 212 Study Guide - Final Guide: Erythrocyte Sedimentation Rate, Osmotic Pressure, Rouleaux
25 Sep 2017
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1 osmotic=one mol of dissolved, non-diffusible, non-ionized substance. In this experiment, we make use of the fact that the number of cells lysing is proprtional is to the hypotonicity of the ecf. The [c] of liberated hemoglobin in each medium is an index of the extent of osmotic hemolysis: more hb in medium, more hemolysis, estimation of the [hb] is done via optical density, which increases linearly with concentration. The od is proprtional to the # of solute particles (hb) in the solution. Rbcs have listed: the zero value for the calibration curve is 0. 9% nacl solution --> no hemolysis, pure distilled water provides the value for 100% hemolysis. The rate at which they settle is measured as the mm of clear plasma present at the top of the column after one hour (mm/hr). In these cases, esr is much > 20 mm/hr.
