BIOLOGY 2B03 Study Guide - Cell Cycle, Leucine, Histone H1

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 2B03
• Professor: Dr, Da Silva, Dr.K.Dej

Document Summary

Objectives: outline the phases of the cell cycle: g1-s-g2-m phase. Identify the functions of key cell cycle regulators: kinases and e3 ubiquitin ligases. Describe their target proteins. q g: examine experiments that identified the activities. Examine experiments that identified the activities of key cell cycle regulators. Cells exit the cell cycle and enter g0. M phase can be further divided into phases: metaphase. 6: prometaphase, prophase, anaphase, telophase h l. Four major classes of cyclin-cdk kinases mitotic mitotic cyclin-cdk. G1-cyclin-cdk l: apc-cdh1, complete m-phase, s-phase inhibitors, transcription factors, prepare for s-phase. G1/s phase cyclin cdk: transcription factors, prepare for m-phase, centromere duplication. M-phase cyclin: chromosome condensation proteins, nuclear envelope proteins, mitotic spindle proteins, kinetochore proteins, apc. Assays: kinase activity (mpf activity): = chromatin y ( y) protein histone h1 phosphorylation, cyclin concentration: antibody to cyclin b, sperm chromosomes monitored for condensation. All contain a homologous sequence near the n-terminus = destruction box or d-box.