BIOLOGY 2B03 Study Guide - Midterm Guide: Protein Targeting, Acetyl-Coa, Secretion

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C. is composed of a serine- lysine- leucine and is found on the c- terminus. Protein a is translated in the cytoplasm and contains a target sequence for a specific organelle. Vesicles are involved in the following transport steps, except for one. Please indicate the pathway that does not use vesicles: trans- golgi to endosomes, er to cis- golgi, trans- golgi to medial- golgi, trans- golgi to plasma membrane. A. sec12: sos, adrenergic receptor, none of the above. Sec18 is a yeast gene that encodes for nsf; mutations in sec18 affect the secretory pathway in yeast. In class we described a famous pulse- chase experiment performed by george. Palade to study how proteins move within the secretory pathway. Definition: proximal signaling, animal cell contact, self- signaling, primordial circulatory system, hormone signaling. Aba signaling in plants is an example of: Short answers: the following is the primary structure of the matrix- targeting signal for transport of a soluble mitochondrial protein.

Related Questions

1. The histones that form the core of the nucleosome get into the nucleus by which of the following?

a) Passive diffusion through nuclear pores

b) Selective and facilitated active transport through nuclear pores

c) Ribosomes located in the nucleus synthetize histones in the nucleus

d) Histones are made at the ER membrane and then diffuse to the nucleus

2. Membrane proteins that belong to the golgi, lysosome, or plasma membrane first pass through which organelle before reaching their final subcellular destination?

a) The golgi apparatus

b) The smooth ER

c) The nucleus

d) The ER membrane

e) The ER lumen

3. Where in the cell would a ribosomal protein be located if it has had its nuclear export signal inactivated (non-functional or non-existent).

a) The protein would be found in the ER lumen

b) The protein would remain in the nucleus

c) The protein would be secreted

d) The protein would remain in the cytoplasm

e) The protein would be in the ER membrane

4. Where would be the final destination of a protein if it contained a NLS anywhere in the protein sequence (and which is exposed to the cytoplasm after protein folding) but also contained an ER signal sequence at the carboxy terminus?

a) The ER lumen

b) The nucleus

c) The cytoplasm

d) The inner nuclear membrane

e) The ER membrane

5. The sequence K-D-E-L serves as a retention/retrieval signal for ER proteins.

a) True

b) False

6. The following are true for both cell membrane proteins and secretory proteins EXCEPT?

a) Precursors of the mature proteins contain signal sequence.

b) Both types of proteins contain stop-transfer sequences

c) Both types of proteins enter the ER while being synthetized

d) Both types of proteins pas through the golgi apparatus

e) Both are carried by clatherin-coated vesicles

7. If you isolate a regulated secreted protein from a secretory/storage vesicle and inject it into the cytosol, is will?

a) Be secreted through channels in the plasma membrane

b) Be taken up into secretory vesicle and secreted

c) Remain in the cytosol until it is degraded

d) Be taken up into the rough ER and follow the secretory pathway

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BIOLOGY 2B03 Study Guide - Midterm Guide: Protein Targeting, Acetyl-Coa, Secretion

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