MBB 222 Study Guide - Final Guide: Ethidium Bromide, Gel Electrophoresis Of Nucleic Acids, Agarose

• School: Simon Fraser University
• Department: Molec Biol & Biochem
• Course: MBB 222
• Professor: Edgar Young

Document Summary

Mbb222 exam vocab definitions: dna electrophoresis and genome structure. Technique commonly used in the lab to separate charged molecules such as. Dna, rna and proteins according to their size. U1-05 load positive vs negative charge; electrode. An electrode is used to produce the positive and negative charge sides. Negatively charged dna moves towards the positive electrode and vice-versa. The gel used consists of a permeable matrix through which charged molecules can travel in when an electric current is passed across it. Smaller molecules will move more quickly and travel further than larger fragments (slower and travel smaller distances) This allows molecules to be separated by their sizes. A compound used in small concentrations for staining so that small quantities of dna can be detected. In gel electrophoresis, agarose gels are used to visualise the fragments of. Different concentrations of agarose are used depending on the size of dna fragments (higher agarose concentration used for a denser matrix)