GENET390 Study Guide - Midterm Guide: Dna Ligase, Sticky And Blunt Ends, Oligonucleotide

• School: University of Alberta
• Department: Genetics (Biological Sciences)
• Course: GENET390
• Professor: George Owttrim

Document Summary

Genet 390 notes from midterm i cut-off to midterm ii cut- off. How to change dna ends: use: to ligate 2 dna"s that initially have non-compatible ends, via, 1) linkers (changing blunt ends into sticky ends) Preparation: make one ont (oligonucleotide) that is palindromic and has re site in the middle of it somewhere. Sometimes linkers link together before being cut. This is no problem because the linkers directly bound to yfg will always be cut and you will be rid of the extra linkers: however . Since yfg is blunt-ended, you cannot stop yfg from ligating to another yfg. Fortunately, this occurs relatively rarely so they would be representative of an extremely small amount of product. The correct product will be most apparent when conducting pcr to test for ligation completeness: 2) adaptors. Are pretty much linkers that contain sticky ends already. Made from a mixture of ssdna and dsdna.