[BIOC 4580] - Midterm Exam Guide - Comprehensive Notes for the exam (94 pages long!)

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Self-assembly & hydrophobic interactions membrane lipids (ii) Biological macromolecules are made up of fundamental building blocks. Building blocks of the cell larger units of the cell. Fatty acids fats / lipids / membranes. Further increase in complexity when looking at how these polymers then react with their. Increase in complexity as you put the building blocks together environments; this is where the omics come into play. Although highly diverse, membrane lipids are all amphipathic. Experimental techniques 1 biotechnical techniques for extracting & analyzing lipids from biological membranes. Biological macromolecules are isolated from biological tissues and cells based on their physiochemical properties. Hydrophobicity is a measurable property of fatty acids & lipids. Partitioning experiment using 2 solvents e. g. heptane and water. Measure partition coefficient: kp = keq = [solute]h2o / [solute]heptane. Calculate free energy change: dgtr = -rt ln(keq) Extraction & analysis of membrane lipids is the basis of lipidomics.

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