MBG 3660 Study Guide - Midterm Guide: Chromosome, Tandem Repeat, Alu Element

• School: University of Guelph
• Department: Molecular Biology and Genetics
• Course: MBG 3660
• Professor: Van Ray

Document Summary

Genetic maps: relative position of two markers based on pedigree studies. If studies show high incidence of co-occurrence then genes will be located close together on map. Based on inheritance and requires at least two alleles and different phenotypes (polymorphism) Based on recombinant frequencies when genes appear close there is low frequency. Co-occurrence: the closer two genes are the more likely they are to be inherited. Genes such as flower colour, disease or adenine dependence in growth. Fragment a sample of dna with restriction enzymes, followed by southern blot, probing and detection of bands. Probe used to determine length of fragments. Markers on either side used to amplify polymorphisms, use pcr and run on agarose gel to determine if homozygous or heterozygous or run capillary electrophoresis to determine stability (high frequency = instable ie. tumourous) Minisatellites: variable number of tandem repeats (vntrs), frequently found at telomeres. Microsatellites: simple tandem repeats (strs), evenly spread around the genome.