BIOL432 Study Guide - Final Guide: Chloroplast, Rna Interference, Trehalose
14 Apr 2015
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19. 2: partially modified to increase transcription or translation within a plant (cid:224) increase 10x, fully modified gene was designed to favour plant codons rather than gram positive bacteria (cid:224) changed so that mrna does not have secondary structure. 2 3% soluble protein in the leaf: by co expressing as part of the same operon a chaperone protein that facilitates correct folding (cid:224) 10 20 fold more protein, inserting into chloroplast genome does not require modification as chloroplast machinery is prokaryotic, many chloroplasts per cell and many copies of chloroplast dna per chloroplast, no unwanted transfer of chloroplast. 2. full length cdna cloned into ti plasmid binary vector and introduced into a. tumefaciens carrying disarmed ti plasmid that contained active vir genes. 2. insects cowpea weevils was found to be proportional to the amount of amylase inhibitor that the transgenic plant produced level of resistance to contained first intron introduced into rice its own promoter and tt was constructed.
