BIO206H5 Midterm: BIO206 Term test 3 potential questions.docx
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Tutorial 5 try to make a diagram which shows all the factors involvedfor all steps and label the major complexes, like pre-initiation, initiation http://bass. bio. uci. edu/~hudel/bs99a/lecture21/lecture2_4. html http://biology. kenyon. edu/courses/biol114/chap05/chapter05. html http://en. wikipedia. org/wiki/genetic_code#start. 2fstop_codons. -> stage 1) initiatior trna (met-trna), and eif2-gtp come together at 40s subunit. mrna with eif4g & eif4e at 5" and other eifs (euk. have more than 10) bind to 40s. Stage 2) when eif4e is recognized by initiator trna, the kozak consensus seq (gcc)a/gccaugg increases ribosomal recognition of aug. Stage 3) atp is used for movement of 40s, eif2 dissociateds when gtp is hydrolyzed and 60s binds with 40s to form the 80s initiation complex. If-3 is released as 50s subunit binds with 30s, and once the 70s initiation complex is formed, other initiation factors (if-1 & if-2) are released. -> stage 1) ef-tu/eef1alpha assemble aa-trna at a-site if anticodon matches (determined by decoding centre at a-site).
