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BIOA02H3 Study Guide - Basil, Herb, Microsoft Excel

Biological Sciences
Course Code
Mary Olaveson

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Comparative Differences In Growth Characteristics of
Basil (Ocimum basilicum) Seedlings: Variety A=
Genovese Basil and Variety B = Rubin Basil.
Hina Javed
Prac: 0002
Bench: 4
TA: Kevin Sam
Tuesday, July 14, 2009

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This experiment is designed to determine whether there is a significant difference
between the growth characteristics of Basil (Ocimum basilicum) seedlings. There are two
varieties of Basil seedlings being used, Variety A = Genovese Basil & Variety B = Rubin
Basil. Basil is known to be a culinary herb which is mainly used in Italian cuisine, but is
also quite famous in countries such as Thailand, Vietnam and Laos. The plant has a
strong taste, sweet smell and is pungent and is usually found in warm & tropical climates.
The herb also has a number of different essential oils which come together in different
proportions for various breeds. (Chalchat and Özcan, 2008)
The question that arises from the background observations of basil properties of different
varieties is that whether or not there are significant differences present between them.
The purpose is to distinguish the seedlings according to their root length, weight and
shoot. The hypothesis for this experiment states that that there are no significant
differences in the attributes of sunflower seeds of both the Variety A=Genovese (Green)
and Variety B=Rubin (Purple). A statistical method called the t-test is used to evaluate the
hypothesis. This enables the experimenter to input all the values obtained at the end of
the experiment into the t- test and evaluate whether the hypothesis is valid or not. These

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values are obtained by taking a sample of measurement of the length of seedling shoots
and the length of the roots; then each seedling is weighed. (Reddy & Kakani 2006), In the
experiment, the data is collected to compare the early growth performance of the two
basil varieties, so the differences in the physical characteristics can be tested for both. If
the seedlings of one variety will show to be bigger in length or weight on average than the
seedlings of the other variety, a difference can be considered between the sizes of the
basil seedlings. And if not, then no difference can be held between the both varieties of
basil seedlings.
Materials and Methods
This experiment used only the basic materials and methods required & the materials used
to conduct the experiment were straightforward. These materials included the seedlings of
two basil varieties, measuring devices such as a ruler, marker, pencil, calculator and a
top-loading balance, as well as Microsoft Excel for the analysis of the data and the
calculations of the statistical values needed to perform the t-test recorded on data sheets.
Also like in any other lab, safety is taken in consideration with wearing of the lab coat and
the safety glasses.
The study was conducted by ~5 students per bench, with each bench given 3-week old
seedlings of Variety A = Genovese Basil with green leaves and Variety B = Rubin Basil
with reddish-purple leaves. Each student determined the size characteristics e.g. weight
and both the shoot and root lengths. The seedlings were gently removed from the soil to
prevent the roots from being damaged. Measurements of both the shoot (above soil
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