# Seedlings formal lab

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University of Toronto Scarborough

Biological Sciences

BIOA02H3

Mary Olaveson

Summer

Description

1
Comparative DifferencesIn Growth Characteristicsof
Basil (Ocimum basilicum) Seedlings: Variety A=
GenoveseBasil and Variety B = Rubin Basil.
Hina Javed
996196753
Prac: 0002
Bench: 4
TA: Kevin Sam
Tuesday, July 14, 2009
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Introduction
This experiment is designed to determine whether there is a significant difference
between the growth characteristics of Basil (Ocimum basilicum) seedlings. There are two
varieties of Basil seedlings being used, Variety A = Genovese Basil & Variety B = Rubin
Basil. Basil is known to be a culinary herb which is mainly used in Italian cuisine, but is
also quite famous in countries such as Thailand, Vietnam and Laos. The plant has a
strong taste, sweet smell and is pungent and is usually found in warm & tropical climates.
The herb also has a number of different essential oils which come together in different
proportions for various breeds. (Chalchat and Özcan, 2008)
The question that arises from the background observations of basil properties of different
varieties is that whether or not there are significant differences present between them.
The purpose is to distinguish the seedlings according to their root length, weight and
shoot. The hypothesis for this experiment states that that there are no significant
differences in the attributes of sunflower seeds of both the Variety A=Genovese (Green)
and Variety B=Rubin (Purple). A statistical method called the t-test is used to evaluate the
hypothesis. This enables the experimenter to input all the values obtained at the end of
the experiment into the t- test and evaluate whether the hypothesis is valid or not. These
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values are obtained by taking a sample of measurement of the length of seedling shoots
and the length of the roots; then each seedling is weighed. (Reddy & Kakani 2006), In the
experiment, the data is collected to compare the early growth performance of the two
basil varieties, so the differences in the physical characteristics can be tested for both. If
the seedlings of one variety will show to be bigger in length or weight on average than the
seedlings of the other variety, a difference can be considered between the sizes of the
basil seedlings. And if not, then no difference can be held between the both varieties of
basil seedlings.
Materials and Methods
This experiment used only the basic materials and methods required & the materials used
to conduct the experiment were straightforward. These materials included the seedlings of
two basil varieties, measuring devices such as a ruler, marker, pencil, calculator and a
top-loading balance, as well as Microsoft Excel for the analysis of the data and the
calculations of the statistical values needed to perform the t-test recorded on data sheets.
Also like in any other lab, safety is taken in consideration with wearing of the lab coat and
the safety glasses.
The study was conducted by ~5 students per bench, with each bench given 3-week old
seedlings of Variety A = Genovese Basil with green leaves and Variety B = Rubin Basil
with reddish-purple leaves. Each student determined the size characteristics e.g. weight
and both the shoot and root lengths. The seedlings were gently removed from the soil to
prevent the roots from being damaged. Measurements of both the shoot (above soil
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portion) and the length of the root (below soil potion) were measured (in mm) and
recorded on the Data Sheet provided. Differences were being noted as any ‘real’
difference would be quite obvious with relatively approximate measurements. The weight
of each of the seedlings was recorded (in g) using the top-loading balance. The class
data was posted on Microsoft excel for the entire class to view on the intranet.
The total seedling length (mm) was calculated by adding both the shoot and root lengths
together. The descriptive statistics e.g. the average, standard deviation, variance,
minimum and maximum values for each seedling characteristic were found by use of the
collective data. Standard deviation was calculated for each of the groups using Microsoft
excel. The deviation was then squared to find variance. The variance was divided by
sample size and then square rooted to calculate the standard error of the mean. These values
were used to calculate the sample t statistics. The value of the sample t statistics was compared
with the critical t statistics, which was obtained from a table. This comparison was made in order
to verify the validity of the hypothewhether the 2 seedling varieties were similar or
different.
Results
Through this experiment the data analyzed publicized the physical characteristics of the basil
seedlings of both Variety A and Variety B as they compare to one another. After the total
length measurements for the two varieties of basil seedlings were graphed, on average
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the seedlings of Variety B are greater in length than the seedlings of Variety A. (62.34mm
vs. 58.11mm) as seen in figure 1.
When the weights were compared of the two varieties of basil seedlings, the averages for
the weight measurements were taken separately for Variety A and B and graphed as
shown in Figure 2. The graphs reveal that on average the seedlings of Variety A have a
greater weight than the seedlings of Variety B. (0.099g vs. 0.05g)
The summary of the results of the measurements, showing standard deviation, both the
minimum and maximum and average values for the two varieties of basil seedlings in
terms of shoot and root length, total length and weight can be found in Table 1. The table
shows that even though Variety A seedlings have greater weight, Variety B seedlings still
have greater values for the total seedling length. As the shoot and root lengths for both
basil seedling varieties were not enormously considerable in their differences.
Finally, two t-tests were carried out and the results were summarized in tables 2 and 3 for
all four test

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