BIO130H1 Study Guide - Scanning Electron Microscope, 3D Reconstruction, Fluorescence Microscope

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13 Apr 2012

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BIO130H1 Full Course Notes

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Microcopy is observing the cell structure and cellular mechanisms. Need to learn how to determine the koehler illumination. Part 1 protocol-adjusting your microscope for koehler illumination. Koehler illumination allows bright and even illumination across a specimen. Begin with x10 objective and turn the microscope on. Open the field iris diaphragm and condenser iris diaphragm. Move condenser up close and slide the condenser focus knob. Close the condenser iris diaphragm until image darkens. Magnification= size of image (mm)/actual size of the penny (mm) Small tiny ruler in the microscope"s oculars. A stage micrometer is usually 1mm in length separated into 100 equal units. Replace your specimen with the stage micrometer slide. Add quieting solution and mix with a toothpick. Take turns looking at the growth of the paramecium. Resolution: distinguish between two closely positioned objects. Limit resolution: is related to the cone of light. Light microscope allows us to magnify cells up to 1000 times.

Related Questions

Briefly, in your own words describe the parts of the microscope.

Base

Light/Mirror

Stage

Condenser

Diaphragm lever

body/head

Nosepiece

Arm

Ocular and objective lenses

QUESTION 1

The ocular of a compound microscope has a magnification of 10x and the objective has a magnification of 10x. The total magnification of the microscope is ___---

	a.	10x
	b.	20x
	c.	100x
	d.	1000x

QUESTION 2

The part of the microscope that focuses the light on an object is the ___________.

	a.	Iris Diaphragm
	b.	Coarse Focus
	c.	Condenser
	d.	Body Tube

QUESTION 3

The polarizing filter in a PLM that is located above the objective is called the _________________.

	a.	Polarizer
	b.	Analyzer
	c.	Abbe condenser
	d.	Iris

QUESTION 4

A substance that reacts the same to light polarized in any direction is _________________.

	a.	an isotope
	b.	anisotropic
	c.	isotropic
	d.	divergent

QUESTION 5

The part of the comparison microscope that allows the examiner to view two objects simultaneously is called the _________________.

	a.	comparator
	b.	comparison bridge
	c.	spectroscope
	d.	stage

1 points

QUESTION 6

A stereo microscope can best be described as _____________________.

	a.	Two compound microscopes aligned so that they each see a slightly different part of an object.
	b.	Two compound microscopes aligned with a comparison bridge.
	c.	A compound microscope with two separate stages and one ocular.
	d.	A compound microscope with two eyepieces and a camera mount.

QUESTION 7

In microscopy, resolution is a measure of ________________.

	a.	The ability of the lenses to separate two tiny details that are close together.
	b.	The total magnification power of the microscope.
	c.	The empty magnification power of the microscope.
	d.	The ability of an electron microscope to determine the presence of a large number of elements.

QUESTION 8

Two polarizers (polarizing filters) aligned 90Â° from each other will block out all light.

True

False

QUESTION 9

A simple convex lens creates a magnified real image of an object.

True

False

QUESTION 10

A trinocular microscope has three objective lenses mounted on a turret.

True

False

QUESTION 11

Liquids and gases are usually isotropic.

True

False

QUESTION 12

Match each of the following terms with Its Definition

Compound microscope a. Has a polarizer and an analyzer

Electron microscope b. Permits viewing two objects at once

Stereomicroscope c. Consists of a single convex lens

Comparison microscope d. Long working distance, three-dimensional

Simple hand magnifier e. Basic 2 convex lens microscope

Polarizing microscope f. Can magnify images more than 100,000 times

maroonmuskrat824

When increasing magnification from high dry to oil immersion,should the iris diaphragm be open or closed? How is this done? Doesthis adjustment increase or decrease the light reaching theobjective?

carmineseal21

BIO130H1 Study Guide - Scanning Electron Microscope, 3D Reconstruction, Fluorescence Microscope

BIO130H1 Full Course Notes

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