BIO130H1 Midterm: BIO130 Section 1 Midterm Notes

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5 May 2016
Simran Singh
BIO130 Section 1 Midterm Notes
Week 4: Lecture 7 (Powerpoint for Week 3 pg 29)
Introduction to DNA Replication:
Lecture Outline:
1. Overview of DNA replication
2. DNA replication in bacteria
3. PCR
Overview of DNA Replication
1. Separate DNA strands
2. Synthesize DNA – with replication
3. Proofread newly synthesize DNA
Ingredients for synthesis:
1. Origin – origin of replication – the
unique sequences that help the
cell know where to being
replication the DNA
2. Primers – DNA polymerase
cannot start replication without a
primer because it needs a free 3’
OH group to add the incoming
nucleotide. – same for rna
3. dNTPs – the building blocks (A, T,
G and C)
4. ATP (as an energy sources)
5. DNA polymerase - enzyme that is
actually putting the nucleotides
6. Accessory proteins - keep DNA
apart once separated – talk about
later in lecture.
DNA synthesis: completely dependent upon base pairing.
Steps in bacterial DNA replication
1. Origin of replication
2. Binding of initiator proteins
3. Unwinding by helicase
4. Binding of single strand binding proteins
5. RNA primers made by primase
6. DNA polymerase
7. Sliding clamp holds polymerase into DNA
8. Nick sealing by DNA ligase (stiches okazami fragments)
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Document Summary

Week 4: lecture 7 (powerpoint for week 3 pg 29) Lecture outline: overview of dna replication, dna replication in bacteria, pcr. Same for rna: dntps the building blocks (a, t, G and c: atp (as an energy sources, dna polymerase - enzyme that is actually putting the nucleotides together, accessory proteins - keep dna apart once separated talk about later in lecture. Initiator proteins for replication in e. coli: binds to origin multiple initiator proteins wrap themselves around the sequence to melt dna - so that dna apart a little bit, helps the helicase bind, requires atp. Then the helicase unbinds just a little bit. After initiator proteins bind for replication in e. coli. Primase binds to helicase (after the helicase has started open the dna strand, the primase comes along and binds along with it ) Primosome the dna helicase and primase together. Rest of the replication machinery can bind then.

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