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Article #6 Summary

2 Pages
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Department
Cell and Systems Biology
Course Code
CSB428H1
Professor
U.Tepass

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CSB428H1F Article #6: Krahn et al.Formation of Baz-Sdt for epithelial polarity
Introduction
Baz phosphorylation site bind PDZ of Sdt to recruit Sdt to PM.
Phosphorylation of Baz causes it to dissociate from Sdt. Non-phosphorylatable Baz blocks
dissociation causing mutation similar to
crb
and
sdt
.
Crb cytoplasmic domain binds PDZ domain of Sdt and Sdt recruit Patj and Lin-7 to apical complex.
Dlg/Lgl/Scrib localize to lateral PM and antagonize Crb-Sdt complex, restricting apical expansion.
BazS980 phosphorylation is required for localization
WT Baz-GFP and Baz
S980E
-GFP shows the same localization basal to Crb-Sdt.
Phosphorylated BazS980-P was concentrated in most apical part, sometimes with/without Crb-Sdt.
WT Baz-GFP and Baz
S980E
-GFP but not Baz
S980A
-GFP rescued maternal and zygotic
baz
lethality.
Overexpression of Baz
S980A
-GFP is similar to
crb and sdt
Overexpression of Baz
S980A
-GFP caused for mation of aggregate containing D E-Cad, Arm,
-cat,
Par-6, aPKC, Crb, Sdt, Patj and Lin7. Lateral marker Dlg was normal.
Baz
S980A
-GFP overexpression caused cells to round up and die of apoptosis.
These dominant-negative effects of Baz
S980A
-GFP were sell-autonomous. Deletion of Baz CR1 or
PDZ domains did not affect is dominancy. However, those lacking PM targeting signal was not
dominant, showing Baz
S980A
-GFP must be at PM to be dominant.
In neuroblasts and oocytes, Baz
S980A
-GFP localize apically like WT and cell fate determinants,
spindle orientation, cell division, and viability was not affected.
Baz
S980A
-GFP mimic crb/
sdt
crb
,
sdt
,
baz
,
aPKC
, and
Par-6
mutants secrete only little scraps of cuticle.
Overexpressing Baz
S980A
-GFP caused a cuticle phenotype similar to
crb
or
sdt
mutants, but
phenotype can be rescued with
lgl
,
dlg
and
scrib
mutation, showing Crb antagonizes Scrib complex.
Overexpressing intracellular por tion of Crb (Crb
intra
) caused ectopic cuticle release.
Overexpressing Baz
S980A
-GFP with Crb
intra
has the same phenotype as overexpressing Baz
S980A
-GFP
1
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Description
CSB428H1F Article #6: Krahn et al. Formation of Baz-Sdt for epithelial polarity Introduction Baz phosphorylation site bind PDZ of Sdt to recruit Sdt to PM. Phosphorylation of Baz causes it to dissociate from Sdt. Non-phosphorylatable Baz blocks dissociation causing mutation similar to crb and sdt. Crb cytoplasmic domain binds PDZ domain of Sdt and Sdt recruit Patj and Lin-7 to apical complex. Dlg/Lgl/Scrib localize to lateral PM and antagonize Crb-Sdt complex, restricting apical expansion. BazS980 phosphorylation is required for localization S980E WT Baz-GFP and Baz -GFP shows the same localization basal to Crb-Sdt. Phosphorylated BazS980-P was concentrated in most apical part, sometimes with/without Crb-Sdt. WT Baz-GFP and Baz S98-GFP but not Baz S980-GFP rescued maternal and zygotic baz lethality. Overexpression of Baz S980-GFP is similar to crb and sdt Overexpression of Baz S980-GFP caused formation of aggregate containing DE-Cad, Arm, -cat, Par-6, aPKC, Crb, Sdt, Patj and Lin7. Lateral marker Dlg was normal. S980A Baz -GFP overexpression caused cells to round up and die of apoptosis. S980A These dominant-negative effects of Baz -GFP were sell-autonomous. Deletion of Baz CR1 or PDZ domains did not affect is dominancy. However, those lacking PM targeting signal was not dominant, showing Baz S980-GFP must be at PM to be dominant. S980A In neuroblasts and oocytes, Baz -GFP localize apically like WT and cell fate determinants, spindle orientation, cell division, and viability was not affected. S980A Baz -GFP mimic crb sdt crb sdt baz aPKC , and Par-6 mutants secrete only little scraps of cuticle. Overexpressing Baz S980-GFP caused a cuticle phenotype similar to crb or sdt mutants, but phe
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