Julius' Basic Rules.pdf

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Department
Immunology
Course
IMM250H1
Professor
Julius
Semester
Winter

Description
Julius'BasicRules • TcellsmatureinThymus ○ Someinintestinalepitheliumsbutrestrictedthere • BcellsmatureinBoneMarrow • IntestinalIntraEpithelialLymphocytes(iLEL) developedTcellshavenoaccesstorestof organism • Lymphocytehomeostasis:BloodandsecondarylymphoidorganshaveroughlyconstantnumberofTandB cells ○ DespitereducedTcellproductionasyougrow • IgawithH-2d • Igbwith H-2b • Allotypespecificantibody(againstanantibody)willnotrecognizeantibodyofadifferentallotype • CongenicStrain ○ AfterF1backcross50% pupsheterozygousforselectedallotype ○ Furthercrossedtocreate1:3 homozygous • 1-(1/2)n ○ n=numberofcrosses • Ab(Ig)producedbyBcells • Abproducedininitialresponsehasloweraffinitythanthoseproducedlater ○ HighdoesofAgactivateshighandlow affinityAbBcells ○ LowdosesofAgactivatesonlyhighaffinityAbBcells • AgconcentrationdeterminesaffinityofAb ○ FrequencyforBcellforanyAb is10 -5 ○ AffinitytobeconsideredAb:~10 5 • Freund'sAdjuvent:MineraloilcontainingAg • Somaticmutation:AfterBcellmaturation • TcellactivationrequiresAPCwithcompatibleMHC • CD4/CD8denotesrestriction-notfunction CD8 MHC I CD4 MHC II • MHC allelesco-dominant • Panexperiment ○ IsolateTcells ○ IncubatewithAPCofcertainMHC allotypeandAg ○ Isolateadheredandnotadhered • MHCLoci MouseStrain K I-A I-E D Class I II II I BALB/c(H-2 ) d/d d/d d/d d/d b C57BL/6 (H-2 ) b/b b/b Chaindefect b/b F1(H-2 )b d/b d/b d/- d/b • 1%of TcellsinpopulationrecognizeallogeneicMHC ○ Tcellsreact to MHC of thespecies(allogeneic)butnottoselfMHC • MHC expressedonthymicepithelium(TE)positivelyandnegativelyselectsTcellrepertoi
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