PCL477H1 Study Guide - Final Guide: Dna Repair, Chek2, Nibrin

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Document Summary

Sensors of the cells tell it what to do- cell cycle transitions, apoptosis, transcription and dna repair. The prototypical sensor dna damage proteins are members of the phoshoinositide 3 kinase related family. 3 are implicated dna damage, atm, atr (rad 3 related) but before these two were discovered we have had dna pkcs gene mutated in schid mice. ** slide 3 man wtf did i write. Dna-pk as a dna damage sensor: phosphorylates p53 on ser15. Phosphorylated p53 is active and acts as a transcription factor: p53 deficient cells lose g1 checkpoint. Dna-pk deficient still has a g1 checkpoint and p53 steady state levels increase in response to damage, Dna-pk is dispensable for activation of g1 checkpoint. Disconnect between dna-pk and p53 and thought (dna pk was the sensor for dsb but maybe its only important for some dna dsb)- thus removable. Gene encoding atm is mutated causes at disease progression. Cells from at patients have hypersensitivity to gamma-irradiation.