Anatomy and Cell Biology 3309 Midterm: Lab 6 - Bone & Bone Formation
22 May 2018
School
Department
Professor
Histology 3309
Lab 6
Identify the tissue:
- hyaline cartilage
o basophilic matrix
o isogenous groups
o pink layer at bottom: perichondrium
Does this tissue have a perichondrium?
- This is an elastin cartilage
o Bc see chondrocytes with lacunae (some isogenous
groups)
o Whisky appearance
- It does have a perichondrium
o Fibrocartilage and articular cartilage don’t have a
perichondrium
Identify the function of this tissue:
- aqua blue is collagen
- this is a fibrocartilage
o linear isogenous groups
- left is 10X magnification
- right is 40X magnification
- found in meniscus, vertebral discs, and pubis symphysis
- function is shock absorption
Identify the two layers of the perichondrium:
- fibrous layer
o has irregular dense connective tissue
- chondrogenic layer underneath
o has chondroblasts
Identify the structure indicated by the yellow circle:
- cell nests (isogenous groups)
find more resources at oneclass.com
find more resources at oneclass.com
Matrix
- 80% water in matrix of cartilage
- in bone, it’s an EVEN distribution of
o water
o organic component
▪ type 1 collagen (causing a lot of
eosinophilia)
o inorganic component
▪ hydroxyapaptite crystals (what
mineralizes the bone)
Cellular Constituents
- mesenchymal stem cells can differentiate into a variety of cells (chondroblasts, osteoblasts,
adipocytes etc)
- in this case, they are going to be differentiating into osteoblasts (which line bone)
- those differentiate into osteocytes (embedded in bone)
- monocyte progenitor cells come together to form osteoclasts
- so we can have multiple cells coming together to form one osteoclasts (so they are
multinucleated)
Osteoblasts
- build bone (undergo appositional growth)
- has a periosteum (has 2 layers)
o fibrous layer
▪ made of dense irregular connective tissue
o osteogenic layer
▪ has osteoblasts that line the surface of bone
- when osteoblasts are active: = cuboidal and basophilic compared to bone
- when osteoblasts are inactive = squamous
- cells can be in an intermediate state (plump and squamous)
find more resources at oneclass.com
find more resources at oneclass.com
