Biochemistry 2280A Final: Bio2280 Final Notes

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Functions or rna: template from which proteins are produced (mrna), along with protein, forming the ribosome and catalyzing protein synthesis (rrna), and carrying amino acids to the growing polypeptide chain during protein synthesis (trna) Components: deoxyribose sugar (missing oxygen an c2) purines: adenine and guanine pyrimidines(single ring): cytosine and thymine. Base pairs lie on inside, phosphates on outside minimizing charge repulsion. 2nm wide, . 34 nm b/w bases, long ~245 million base pairs in human chromosome. Stabilization: base stacking: non-specific stacking of planar rings enables hydrophobic interactions (van der waals, base pairing: sequence specific h bonds between c g (3 bonds) and a t (2 bonds), resulting in constant backbone distance. Polynucleotides are written 5" (phosphate) 3" (free hydroxyl) Definitions: chromatin - the complex of dna and its organizing proteins histones small highly conserved proteins (<200 aa) with many arginine and lysine residues (basic residues), making them positively charged and ideally suited to interact with negative.

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