Biology 2581B Study Guide - Quiz Guide: Spindle Pole Body, Polymerization, Cryogenic Transmission Electron Microscopy
3 Jun 2020
School
Department
Course
Professor
Document Summary
Nucleation in microtubules: themselves polymerise enriched (found in all known mtocs: easily visualised by em protofilaments in parallel, gcps 2-6, proteins = gamma complex proteins (gcps) w. increasing mw, also well conserved. Spindle body: yeast nucleation from mtoc embedded in nuclear envelope spindle pole, multiple forms only complexes can nucleate mt formation. Get structure cryo-em high res. Much greater polymerisation rate in vivo than vitro suggest necessary assembly: forms gamma tubulin ring complex template of microtubule w. 13 i. e. individual subunits not effective nucleators need complex formation. Like the monomer but much larger (reflect 13 protofilament polymer: like arp2/3 addition to template (mimic) Rds but additional instability of resultant polymer curved filament - stress catastrophe / rescue stochastic transition between. D form promotes curved hydrolysis on incorporation. T from promotes linear hence t cap promotes linear association @ cap. Nucleation localised to specific complexes microtubule organising complexes. Gamma tusc small complex: themselves may oligomerise > ring like need spc110.
