Biology 1002B Study Guide - Midterm Guide: Enzyme Kinetics, Carbon Fixation, Chlamydomonas

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1. change in respiration rate (oxygen consumption) in isolated mitochondria by addition of nadh, adp, uncoupler etc. 2. definition of respiratory control and how it is accomplished (proton gradient). 5. reasons why chlamydomonas can grow as a heterotroph on certain reduced carbon compounds - but not others. 2. how one can distinguish between gas exchange in mitochondria from that taking place in the chloroplast of a chlamydomonas cell. 3. identify major parts of a light response curve for carbon fixation. 4. what metabolic processes and external factors may influence the change in rate as a function of light. 6. principal of measuring enzyme kinetics as a function of substrate concentration. 7. concept of saturation kineticsin enzyme reactions and transport. 8. features that differentiate simple diffusion and facilitated diffusion. km, vmax. 9. effect of a competitive inhibitor on enzyme kinetics (vmax, km) 10. role of allosteric activation and inhibition in the regulation of metabolism. 2. identify the paradox for why prokaryotes are biochemicall complex don"t develop morphological complexity.

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