Biology 2290F/G Study Guide - Midterm Guide: Wee1, Apolipoprotein, Familial Hypercholesterolemia
3 Mar 2014
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Media which contains amino acids, vitamins, minerals, salts, sugars; all the nutrients the cell would normally have within the body. Serum which contains insulin and growth factors; helps cell take in sugars, and keeps cell growing and dividing respectively. Temperature of 37o c and the culture is grown in a co2 incubator; incubator necessary to mimic the gas ratio as it is in the body---need to balance co2 and o2 as it is in the body. The culture should be coloured red; colour is a ph indicator---yellow would be too low ph, purple would mean too high ph. Primary cell cultures are taken directly from an organism, they have a limited number of times that they can divide (hayflick limit) due to formation of confluent monolayers as a result of contact inhibition. Still have regulatory mechanisms that tell the cell to stop dividing or kill itself.
