Physiology 3140A Study Guide - Quiz Guide: Short Hairpin Rna, Plasmid, Transfection

• School: Western University
• Department: Physiology
• Course: Physiology 3140A
• Professor: John Di Guglielmo

Document Summary

Assignment 4 - rnai *****videos not included***: rnai are any rnas that can induce silencing of other molecules. Can be split into two types: sirna and microrna: rnai was discovered through the microinjection of double-stranded rnas (dsrna) into c. elegans. Within the successfully injected cells: the complementary mrna of the injected dsrnas are not expressed in protein. Demonstrated that there are endogenous mechanisms to repress mrna translation in eukaryotes = rnai pathway. Short interfering rna (sirna): the proteins in the rnai pathway that recognize & cleave dsrna into shorter pieces. Other proteins exist that uses sirnas to target speci c mrna sequences in the cytoplasm = once cleaved, tagged for degradation. A method for gene transcription regulation & laboratory experiments: silencing machinery exists to combat foreign viral dsrna genome invasion. Humans encode micrornas that uses the same silencing machinery as sirna in c. elegans = control what mrna are translated, a genome regulation.