BIOL 1000 Study Guide - Midterm Guide: Genetic Recombination, Phosphodiester Bond, Restriction Enzyme
13 Nov 2016
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Biotechnology: biological for industrial & other purposes. The genetic manipulation of microorganisms to produce antibiotics, hormones. Ligation: covalently linking the two ends of dna or rna molecules by an enzyme like dna ligase, rna ligase. It i(cid:374)(cid:448)ol(cid:448)ed (cid:272)reati(cid:374)g a phosphodiester (cid:271)o(cid:374)d (cid:271)et(cid:449)ee(cid:374) the 3" h(cid:455)dro(cid:454)(cid:455)l of o(cid:374)e (cid:374)u(cid:272)leotide & 5" phosphate of a(cid:374)other. Transformation: is the genetic alteration of cell because of an uptake of foreign genetic material. Bacterial can be formed by introducing new gene sequences in their plasmids. A bacterium is considered transformed if it represents and reflects the new genes. Digestion: it"s a pro(cid:272)edure used i(cid:374) (cid:373)olecular biology to digest dna into smaller fragments for further analysis. It can be used for cleaving molecules at certain sites. Enzymes called restrictions enzymes are responsible for this cleaving of the dna. Restriction enzymes: an enzyme having the property of cleaving dna molecules at or near specific sequences of bases.
