BIOL 2020 Study Guide - Midterm Guide: Ethidium Bromide, Glycosidic Bond, Acridine Orange
24 Apr 2015
School
Department
Course
Professor
Document Summary
Label of entire molecule if more than 1 chiral center is by using the furthest chiral c from the c=o: disasteromers: non-mirror image isomers. Epimers: orientation differs only at a single chiral c. C1: glycoslation reaction: attach sugars to proteins/lipids by the anomeric (c1) carbon, catalyzed by glycosyl transferase, glycoprotein, o-glycosidic bond to ser or thr, or oh on membrane lipids, n-glycosidic bond to asn. H bonds + base stacking keeps strands together. Rna: extra oh on ribose = less stability. 2ndary structure: single strand, some complementary portions can hybridize and make local double strands: hairpin, bulge, internal loop, helical segment trna: common 2ndary structure, 5" overhang: 3" strand is longer ( overhang with 5" exposed) 3" overhand: 5" strand is longer (overhand with 3" exposed) Restriction digestion -> analyze by agarose gel electrophoresis + ethidium bromide stain -> id sample based on segments. Ecori: gaattc, cuts 3" to to g, type a endo for dna (sticky)
