BIOL 1020 Study Guide - Final Guide: Dna Replication, Dna Ligase, Histone H1
BIOL1020 Exam Three Questions from Previous Tests
1. Histone H1 interacts with linker DNA to form the
a. Packed nucleosomes (30 nm fiber)
2. Translation ends when a stop codon is at the ____ site, allowing a/an ______ to
enter there and then cause the translation machinery to fall apart and release
the new polypeptide.
a. A site; termination factor
3. There are 61 mRNA codons that specify an amino acid, but only 45 tRNAs.
This is best explain by the fact that
a. The rules for base pairing between the third base of a codon and tRNA
are flexible.
4. Meselson and Stahl found evidence supporting the theory that DNA
replication is semiconservative when they grew bacteria in a medium
containing “heavy” nitrogen and then transferred them to a medium
containing N-14. Which of these results in the figure above matches the
results that they found after TWO rounds of DNA replication in the presence
of N-14?
a. E
5. The polypeptide SJK has 68 amino acids. The mutated version of SJK
polypeptide has only the first 32 amino acids. What kind of mutation could
have caused this?
a. Two of the above
6. Refer to the representation of DNA replication in Figure 16-1 above. The
structure labeled as “D” is:
a. Single-strand DNA binding protein
7. Refer to the representation of DNA replication in Figure 16-1 above. The
structure labeled “A” is:
a. DNA ligase
8. Refer to the representation of DNA replication in Figure 16-1 above. The
structure labeled “B” is
a. DNA polymerase
9. RNA polymerase moves along the template strand of DNA in the ____
direction of the template strand, and adds nucleotides to the ____ end of the
growing transcript.
a. 3’ to 5’ direction; 3’
10. Prokaryotes perform transcription in the _______ and translation in the
________.
a. Cytoplasm, and cytoplasm
11. The cap on an mRNA is
a. Required for binding to a ribosome in eukaryotes
12. Human cells produce perhaps as many as 100,000 different proteins, and yet
the human genome has only about 25,000 genes. This is best explained by
a. Alternative splicing
13. Which of the following represents the order of increasingly higher levels of
organization of chromatin?
a. Nucleosome, 30-nm chromatin fiber, looped domain
14. During DNA replication, the ______ strand is assembled in the ________ direction
that the DNA double helix unwinds and is produced by __________ replication.
a. Lagging strand; opposite direction; discontinuous replication
15. This type of RNA forms the main structural and catalytic elements of
ribosomes
a. rRNA
16. Muscle cells and nerve cells in one species of animal owe their differences in
structure to
a. Differences in gene expression
17. During DNA replication, DNA ligase is most active on the lagging strand. This
is because:
a. The lagging strands contain more short DNA segments than the
leading strand, and these short segments are joined together by DNA
ligase
18. Eukaryotic telomeres replicate differently than the rest of the chromosome.
This is a consequence of which of the following?
a. Gaps left at the 5’ end of the lagging strand because of the need for a 3’
onto which nucleotides can attach
19. In the Hershey and Chase experiment, the pellet was radioactive after
bacteria had been infected with P-32 labeled viruses and centrifuged. Why
a. Bacteria were centrifuged to form the pellet, and they had
incorporated radioactive DNA.
20. The process of transcription refers to the direct use of information encoded
in _____ to make ________.
a. DNA; an RNA strand
21. The central dogma describes the flow of information of gene expression as:
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BIOL 1020 Full Course Notes
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Document Summary
Which of these results in the figure above matches the results that they found after two rounds of dna replication in the presence of n-14: e, the polypeptide sjk has 68 amino acids. The mutated version of sjk polypeptide has only the first 32 amino acids. What kind of mutation could have caused this: two of the above, refer to the representation of dna replication in figure 16-1 above. The structure labeled as d is: single-strand dna binding protein, refer to the representation of dna replication in figure 16-1 above. The structure labeled a is: dna ligase, refer to the representation of dna replication in figure 16-1 above. ________: cytoplasm, and cytoplasm, the cap on an mrna is, required for binding to a ribosome in eukaryotes, human cells produce perhaps as many as 100,000 different proteins, and yet the human genome has only about 25,000 genes.
