BISC104 Study Guide - Final Guide: Taq Polymerase, Polymerase Chain Reaction, Gel Electrophoresis
31 Oct 2018
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No two individuals are genetically identical (except identical twins) Small differences in nucleotide sequences of their dna. Compare sizes of these markers (compare pattern of bands) Quickly results in billions of copies of target sequence. 1 copy 2 copies 4 copies 8 copies 16 copies (etc) As # of repeats increase, length of segment increase. Gel electrophoresis separates dna molecules by size. Each person will have unique patterns of bands. The flow of genetic information in the cell. Carries genetic information out of nucleus into cytoplasm. The flow of genetic information in a cell is dna rna protein. One of the two dna strands gets chosen to be transcribed into rna, and then gets translated to protein. Production of rna using dna as a template. Rna nucleotides complementary base pairing to dna. T a, c g, g c, a u. No thymine in rna, turns to a u. Amino acids needed for protein synthesis (in ribosomes)
