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Photoseries Six Study Guide

6 Pages
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Department
Neuroscience
Course Code
NROB60H3
Professor
Janelle Leboutillier

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Photoseries 6 - Coronal Sections
Anatomy of Dissection
In the following dissection, ten coronal cuts will be made along the length of the brain. Of the first seven
cerebral cuts, only three are necessary; however all seven should be conducted for better understanding
of structures and how they traverse through the brain (for example, the development of internal
capsule from fibres at the caudal end to tracts as we approach the middle). The premier cuts, C, D, and
E, are referred to as rostral cerebral coronal dissections and these outline the head of caudate as well as
the olfactory region. The following cuts, F and G, outline the middle cerebral coronal dissections and the
thalamic nuclei as well as fimbria of hypothalamus emerge here. The caudal cerebral coronal
dissections, H and I, consist of the geniculate bodies, substantia nigra, and the remaining structures of
hippocampus. Lastly, the cerebellar cuts (J, K, and L) have a wide variety of structures unique to each
cut. These cuts are best performed with two people in general; however the cerebellar cuts should be
performed by one person to avoid any close encounters with fingers and knives.
Material List
x Dissection tray
x Large knife (for cuts through)
x Scalpel (for finer incisions, separations, and excision of meninges unattended during first lab)
Procedure of Dissection
1. First, invert the tray such that the flat gray underneath is facing up
2. Place the brain on top the setup such that the ventral side is visible (Figure 1)
Figure 1 Ventral surface of Sheep Brain
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3. To attain the first necessary dissection, one person should hold the brain embracing the
cerebellum between their thumb and index plus middle finger while the other aligns the large
dissection blade just anterior to the optic chiasm (C, D, & E) (cut is through the olfactory tracts)
~&]PµîX<Zlv](v]µo}ZÇ[µ(vPvoÇUµ(]uoÇµZ]PZ
down.
Figure 2 First cut through olfactory tract
4. As the blade of the knife touches the tray, slowly draw back the knife towards you (the contact
between the knife and tray should be maintained) and let the two halves separate naturally (use
scalpel to make any minor alterations for such motion to occur). The final division should be
similar to Figure 3, showing the caudal structures.
Figure 3 Final look of the first cut
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Description
Photoseries 6 - Coronal Sections Anatomy of Dissection In the following dissection, ten coronal cuts will be made along the length of the brain. Of the first seven cerebral cuts, only three are necessary; however all seven should be conducted for better understanding of structures and how they traverse through the brain (for example, the development of internal capsule from fibres at the caudal end to tracts as we approach the middle). The premier cuts, C, D, and E, are referred to as rostral cerebral coronal dissections and these outline the head of caudate as well as the olfactory region. The following cuts, F and G, outline the middle cerebral coronal dissections and the thalamic nuclei as well as fimbria of hypothalamus emerge here. The caudal cerebral coronal dissections, H and I, consist of the geniculate bodies, substantia nigra, and the remaining structures of hippocampus. Lastly, the cerebellar cuts (J, K, and L) have a wide variety of structures unique to each cut. These cuts are best performed with two people in general; however the cerebellar cuts should be performed by one person to avoid any close encounters with fingers and knives. Material List N Dissection tray N Large knife (for cuts through) N Scalpel (for finer incisions, separations, and excision of meninges unattended during first lab) Procedure of Dissection 1. First, invert the tray such that the flat gray underneath is facing up 2. Place the brain on top the setup such that the ventral side is visible (Figure 1) Figure 1 Ventral surface of Sheep Brain www.notesolution.com 3. To attain the first necessary dissection, one person should hold the brain embracing the cerebellum between their thumb and index plus middle finger while the other aligns the large dissection blade just anterior to the optic chiasm (C, D, & E) (cut is through the olfactory tracts) ~]2µŒî:‰šZlL]‰Œ‰L] µoŒš}šZšŒÇ[ZZµŒ L2LšoÇ7µš]ŒKoljµZZZšŒ]2Zš down. Figure 2 First cut through olfactory tract 4. As the blade of the knife touches the tray, slowly draw back the k
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