BIO130H1 Chapter Notes - Chapter 18: Restriction Enzyme, Endonuclease, Peptide
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BIO130H1 Full Course Notes
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Document Summary
Over the past 30 years, tremendous advances have been made in the analysis of eukaryotic genomes. This progress began as molecular biologists learned to construct. Dna sequences derived from more than one source. Recombinant dnas can be used in myriad ways: one of the most important applications is the isolation from the genome of a particular segment of dna that encodes a particular polypeptide. First, however, it is necessary to consider a class of enzymes whose discovery and use has made the formation of recombinant. During the 1970s, it was found that bacteria contained nucleases that would recognize short nucleotide sequences within duplex. Dna and cleave the dna backbone at specific sites on both strands of the duplex: these enzymes are called type. Endonucleases , or simply restriction enzymes: they were given this name because they function in bacteria to destroy viral dnas that might enter the cell, thereby restricting the growth of the viruses.
