BIO130H1 Chapter Notes - Chapter all assigned: Transcriptome, Coiled Coil, Comparative Genomics
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BIO130H1 Full Course Notes
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Transcriptome: all rna in cell/tissue at time proteome: proteomics: interactome: metabolome: phenome: genome: dntp: Fish: chromatin: nucleosome: to paint chromosomes, make probe complementary to target dna w/ fuorescent probe tightly packaged (but not chromosomes) dna + proteins (histones); Ssb: ssdna: replisome: helicase + primase holds dnapiii onto strand ( is processive moves along quickly ) Dnapiii attached in front of it single-stranded binding protein single-strand dna binding protein in e. coli area around replication fork with all associated proteins. Dna fragment + rna primer telomere: telomerase: repetitive sequence (ttgggg g-rich!) at ends of chromosomes, access points for rna primers has built-in rna template, adds complementary dna to 3" parental strands; has a reverse transcriptase subunit adds g-rich. Cpsf (cleavage & polyadenylation specificity factor) and cstf (cleavage stimulating factor) poly-a-binding protein (pabp): coats poly-a-tail after poly-a-polymerase adds 200-250 as nuclear exosomes: degrade improperly processed mrna reading frames: 3 reading frames per mrna aminoacyl-trna synthetases: attach aas to correct trna (20)
