BCH210H1 Chapter 2: R2

• School: UTSG
• Department: Biochemistry
• Course: BCH210H1
• Professor: Stavroula Andreopoulos

Document Summary

We can then overlap the peptide fragments which can be used to resemble the complete sequence: Aaarfaaaklaaamaaaa: protein (peptides) bombarded by high energy electron beams or laser beams, this will cause the proteins to ionize, which impact upon a charged plate: Depending on how charged they are; they will move toward the plate at different speeds which will detect its exact molecular weight. Ms is a technique used to precisely detect the molecular weight of a protein/peptide based on mass & charge ratio. Turns are very short which are fixed; can be observed in the x-ray structure. Loops are long which are disordered/mobile/dynamic; can"t be observed in the x-ray structure: can observe the 3 structure. Crystallographic data: the folding of the protein backbone can be specified precisely, the ( , ) angles of each residue allows us to know the 2 structures, the position (coordinates: x, y, z) of every atom in 3d.