Biochemistry 2280A Chapter Notes - Chapter Topic 5: Fertility Medication, X-Ray, Tandem Mass Spectrometry
24 Sep 2016
School
Department
Course
Professor
Document Summary
Proteins can be purified from cells or tissues: Cell homogenate or extract: break cells and release contents. Chromatography: utilizes different materials to separate the individual components of a complex mixture into portions or factions: on the basis of binding particular molecule: affinity chromatography. Electrophoresis: separate proteins to see size and net charge. Determining a protein"s structure begins with determining its amino acid sequence: They can be analyzed by a secondary spectrometer: typically used for protein complexes, x ray crystallography or nuclear magnetic reconance spectroscopy can be used to find folding patterns of proteins. Genetic engineering techniques permit the large scale production, design, and analysis of almost any protein: We can make proteins but need products from tissues, sampes (urine for fertility drugs) The relatedness of proteins aids the prediction of protein structure and function: databases from grunt work can be accessed now, similar sequence patterns typically = same protein families, typically fold into same structural domains, less single domains.
