Biochemistry 2280A Chapter Notes - Chapter Genomes 3: Plasmid, Contig, Agarose Gel Electrophoresis
24 Dec 2016
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Shotgun method: look at genome and then look for overlaps to build master sequence: standard for small prokaryotic genomes, 2 problems, difficult and more complex the larger the genome. Possible over lap increases by 2n: can lead to errors in repetitive sequences. Can even lead to sequences from different chromosomes being linked/associated together on accident. Shotgun cannot be used on large molecules until we have a genome map: an idea where the genes are and traits are. Whole genome shotgun method: large genomes are mapped with shotgun method but with a genome map, we have information on genes and whatnot to ensure that when we are assembling, we are assembling correctly. Genetic mapping: genetic techniques to show position of genes: cross breeding, examine of pedigrees. Probe the unstable sequence with oligonucleotides of the sequence contigs. Primer the oligonucleotide and see if there is a pcr product with another oligonucleotide.
