BIOL 2021 Chapter Notes - Chapter 13.10: Copii, Clathrin, Hydrolysis

• School: York University
• Department: Biology
• Course: BIOL 2021
• Professor: Patricia Lakin- Thomas

Document Summary

Arf-gap becomes activated when curvature approaches shape of transport vesicle which it then inactivates arf, causing coat to disassemble. Gap activates to cause the inactivation of arf protein (responsible for assembly of coat proteins). Arf-gap is recruited to copi coat as it assembles. Arf-gap becomes activated when curvature approaches shape of transport vesicle which it then inactivates arf, causing. Inactive, soluble sar1-gdp binds to a sar1-gef in er membrane, causing sar1 to release to release its gdp & bind gtp. A gtp- triggered conformational change in sar1 exposes an amphiphilic helix, which inserts into cytoplasmic leaflet of er membrane, initiating membrane bending coat to disassemble. So arf-gap activates to cause the inactivation of. Arf protein (responsible for assembly of coat proteins). Gtp-bound sar1 binds to complex of two copii adaptor coat proteins, which form inner coat. the entire surface of complex that attaches to membrane is greatly curved, Membrane-bound, active sar1-gtp recruits copii adaptor proteins to membrane.