GEN-3000 Chapter Notes - Chapter 17: Cloning Vector, Molecular Cloning, Recombinant Dna
13 Dec 2017
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Recombinant dna technology: molecular technique for locating, isolating, altering and studying dna segments. Allows manipulation of different dna sequences that come from different sources. Biotechnology: use biosystems, living organisms or their derivatives to make/modify products or processes for specific use. Restriction enzyme (re): recognizes specific nucleotide sequences and makes cuts. Need one re copy on vector so vector doesn"t get diced up. Can be read the same when read forward or backward. Single strands like to form hydrogen bonds w/ others. Enzyme doesn"t care about where dna is from. As long as the molecules are cut by same enzyme. Cloning vector: dna molecule that can be attached to piece of foreign. Use cloning when: want large amount of dna for experiment. Make sure: a) plasmid got into the cell and b) the desired dna of choice got into the plasmid. Use transformation to get desired dna fragment into bacteria cells. Start with cloning vector a. b. c. d.
