GEN-3000 Lecture Notes - Lecture 17: Recombinant Dna, Molecular Cloning, Restriction Enzyme
7 Dec 2020
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D n a created by joining together pieces of d n a from various. Allows for isolation and study of specific d n a sequences. sources (recombinant d n a: clones: recovered copies of recombinant d n a molecule: Used to study structure and orientation of d n a. Tools of recombinant d n a technology: two important tools used to construct and amplify d n a molecules: Restriction enzymes: d n a-cutting enzymes. Produced by bacteria as defense mechanism against infection by. Restrict or prevent infection by degrading d n a of invading virus. Bind to d n a at specific recognition sequence (restriction site) Enzyme cleaves both strands of d n a (digestion). and cuts d n a to produce restriction fragments (figure 17-1). Common restriction enzymes, with their recognition sequence, d n a cutting patterns, and source microbes. Arrows indicate the location in the d n a cut by each enzyme.
