BIOL 1201 Chapter : Biology Chapter 16 The Molecular Basis Of Inheritance

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azuremammoth471

15 Mar 2019

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Replication begins at a site called origin of replication. [in bacteria, this is recognized by replication enzymes. ] Rna (primer) , provides free 3" oh of primer and intiates synthesis. Polymerase iii: leading strands: synthesize continuously lagging strands: synthesize discontinuously in small fragments called. The replication enzymes will seperate the double stranded dna, generating a replication bubble. Dna polymerase iii: recognizes 3" oh end of nucleic acid. Helicase binds at the replication fork and unwinds the dna double helix. Single stranded dna binding proteins (ssbp): binds to ss dna & prevents dna from annealing. Dna polymerase i: removes primers/replacing it with dna, and leaves behind a broken phosphodiester bond called a nick. Each daughter molecule will have one old strand (derived or. Conserved from the parent molecule) and one newly made strand. Dna are packaged into a structure called chromatin (a complex of dna and proteins) which condenses to form a chromosome. Usually only one copy of chromosome per cell.

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1) What is the difference between the leading strand and the lagging strand in DNA replication? Place the following steps of DNA replication in right chronological order.

1. Primase adds an ribonucleotide primer
2. Initiator proteins bind the origin of replication and helicase breaks hydrogen bonds between complementary bases of double stranded template DNA
3. DNA polymerase III extends by complementary base pairing deoxyribonucleotides to the template strands
4. Ligase phosphodiester bonds the nucleotides to connect okazaki fragments nad connect fragments at the origin and where the "crotches or forks" collide
5. DNA polymerase I removes RNA primer and replaces with Deoxyribonucleotides

2) Given the template DNA and primer sequences below, what bases will be added to the primer as DNA replication proceeds? (The bases should appear in the order that they will be added)

5'-GTCGTCTCCCTTTTTAAAAACCCCCCC-3'
5'-UUUUUAAAAAGGG-3'

3) Given the DNA sequence below, what could be a primer sequence used in its replication?
5'-CGTACGGCCCCAAAATTGCTACTCTGCTG-3'

raspberryleopard304

Which of the following are required for DNA replication by DNA polymerase?

A. Template strand

B. Short, complementary primer strand

C. 5'-dXTPs

D. 3'-dXTPs

E. Answers (A) and (C)

F. Answers (A) and (D)

G. Answers (A), (B), and (C)

H. Answers (A), (B), and (D)

Which of the following are reasons why DNA replication must be discontinuous?

A. The complementary strands have opposite polarity

B. Only 5'-dXTPs are available in cells

C. DNA polymerase only adds 5-'dXTPs to 3'-OH ends

D. All the above

E. Answers (A) and (C)

F. Answers (B) and (C)

G. Answers (A) and (B)

Which of the following are roles of RNA polymerases in DNA replication?

A. RNA polymerase (primase) initiates replication at the origin

B. RNA polymerase synthesizes the primers for Okazaki fragments

C. Both (A) and (B)

D. Neither (A), nor (B)

Which of the following statements describes the activity of DNA polymerase?

A. DNA polymerase adds 3â-dXTPs to the 5â-OH terminus of the primer
B. DNA polymerase adds 5'-dXTPs to the 3â-OH terminus of the primer
C. DNA polymerase adds 5â-dXTPs to the 3â-phosphate terminus of the primer
D. DNA polymerase adds 3â-dXTPs to the 5â-phosphate terminus of the primer

oliveraccoon574

Double check to see if I got these right? 3 questions

Some Processes Involved in DNA Replication

1.The replication origin is identified.

2 DNA primase builds RNA primer.

3Okazaki fragments are spliced by DNA ligase.

4Helicases bind and uncoil the DNA double helix.

5 RNA primer is the starting point for DNA polymerase.

6 DNA replication ends at the telomere where DNA codes fortermination.

7 DNA polymerase adds complementary nucleotides in the 5' to 3'direction in short segments called Okazaki fragments.

The sequence in which the events numbered above occur during DNAreplication of the lagging strand: Number 1 - 7 in order.

I put: 1,4,2,5,7,3,6

__________________Question 2:

Some Functions of Enzymes

Build DNA strands
Build RNA primers
Build mRNA strand
Cleave DNA strands
Splice Okazaki fragments

Enzyme:
Helicase :4
DNA ligase :5
RNA primase :1
RNA polymerase :3
DNA polymerase:2

Question 3:

like mRNA, tRNA has a ribose sugar, U instead of T, and issingle stranded. Unlike mRNA, which remains a long single strand ofnucleotides, tRNA folds so that some areas pair up.

The resulting structure has an anticodon on one end and a site foran amino acid to attach on the other end. There is basecomplementarity (A pairs with U and G pairs with C) between an mRNAcodon and tRNA anticodon.

If the amino acid tyrosine attaches to a tRNA, which of thefollowing anticodons could be at the opposite end of the tRNAmolecule?

Select one:

a. ATA and ATG

b. UAU and UAC

c. AUA and AUG

d. AGA and AGU

I picked B.

purpleturtle13

BIOL 1201 Chapter : Biology Chapter 16 The Molecular Basis Of Inheritance

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