BIOL 4246 Chapter : 6 19 12 Translation
Document Summary
Codon preferences- those for which the organism has the most trna. If you want a highly expressed protein you have preferred codons throughout the whole protein. Alternate codon assignments- exceptions to the rule- most common is stop codons used for amino acids. Selenocysteine inserted at the stop codon if you have the rna secondary structure that recognizes it and allows it to insert. There are 61 codons for 20 amino acids leads to degeneracy of the code. Every amino acid has more than one codon except for: methionine, styptophan, selenocysteine or pyrrolysine, utilize the wobble position in the anticodon to mediate with the degeneracy. 3" base of the codon bp with the 5" b of the anticodon (trna) 3" end of trna always ends with cca. Even if it"s not genetically encoded it will be added on. Had a lot more diverse trna genes than the first e. coli.
