BIOL 111 Chapter Notes - Chapter 6: Cellular Respiration, Peptide, Phagocytosis
Document Summary
Microscopy used to study very small cells and organelles. Microscopes used by renaissance scientists, robert hooke= father of microbiology. Light microscope- visible light is passed through the specimen and then through a glass lens: brightfield, phase contrast, differential-interference contrast, fluorescence, confocal, deconvolution, super-resolution. Electron microscope- focuses a beam of electrons through the specimen or onto its surface so an image can be projected onto the microscope. Scanning em- useful for detailed topography of a specimen, beam excites electrons on the surface of the specimen, result is an image that appears 3d. Transmission em- aims electron beam through section of a specimen stained with atoms of heavy metals, electrons scattered into denser regions, image displays patterns of transmitted electrons. Disadvantages of em: methods to prepare the specimen, kill the cells. Light microscopy has been revitalized by major technical advances. Increased detail and resolution, confocal and deconvolution microscopy have sharpened images of 3d specimens.
