BIOL 2P98 Lecture Notes - Lecture 4: Gram-Negative Bacteria, Autoinducer, Cell Membrane
30 Jul 2015
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Industrial, health, and research considerations: growth; chemical & physical factors. 2: for example: imagine you are hired by pfizer to help develop a new antibiotic from a strain of bacillus. You have one 10,000 gallon tank available, costs /day to run. You must decide how much inoculum to put in tank, when to harvest bacteria for maximum profit, minimum cost. What numbers do you need: another example. You are hired as a research assistant in a laboratory. You need to grow bacteria to a concentration of 5 x 108 cells/ml, then harvest these cells for use in an experiment. Batch method: put small inoculum of pure culture into sterile medium, let grow. Common lab procedure, but not typical of many real environments. Trickle fresh medium into culture at slow but steady rate, displace = volume of culture as overflow. Cells remain in exponential (but suboptimal) state, growing at known rate. Good simulation for study of many natural environments.
