BIOL 3P50 Lecture Notes - Lecture 6: Reagent, 2-Mercaptoethanol, Affinity Chromatography
27 Aug 2015
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Document Summary
Identification of protein-coding: follows assembly sequences, regulatory rnas, etc, assign potential or known functions to genes when possible structures. Identification of genes is difficult for animal genomes with complex intron-exon. Rnas: comparative genomics to identify regulatory sequences. Whole genome tiling microarray: one oligonucleotide is spotted on the array for every. ~150 bp of genomic dna in a sequential manner: hybridize with fluorescently labelled rna or cdna to detect expressed sequences, overlay with genome information to verify protein- coding sequences (exons), and non-coding rnas. Identification of enhancers through sequence analysis: genomic sequence can be scanned for potential binding sites of known regulatory proteins, usually recognize short sequence motifs, e. g. 6 10 bp. Coomassie blue: total protein can be visualized by incubating the gel with coomassie blue reagent, binds to protein non-specifically, protein appears as blue bands in gel. Detection of a single protein in a sample via western blot actual western blot.
