BIOL 461 Lecture Notes - Lecture 7: Antisense Rna, Dicer, Andrew Fire
12 Feb 2018
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Biol 461 lecture 7 - stop at slide 42 how we obtain mutants for genetic analyses. Quick review of transcription/translation: dna in the nucleus is transcribed by rna polymerase making an. Rna transcript which is spliced in the nucleus and capped: transported out to the cytoplasm where its translated by a ribosome to make the polypeptide which is the protein which folds into the protein that makes the function. Scientists were interested in knocking down the expression of genes. Did(cid:374)"t (cid:449)o(cid:396)k (cid:271)ut lead to the dis(cid:272)o(cid:448)e(cid:396)(cid:455) of something else. P(cid:396)otei(cid:374) that"s (cid:396)e(cid:395)ui(cid:396)ed fo(cid:396) (cid:374)eu(cid:396)o(cid:373)us(cid:272)ula(cid:396) control in the worm. If they injected ds-rna for that specific gene then they generated the mutant phenotype (twitching movement) Injecting dsrna could disrupt the expression specifically of a gene. Dsrna is a sequence from the mex-3 rna. Target rna is gone, and all the other rnas are fine. In-situ hybridization to detect the mrna in the embryo.
